Metabolic Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Inhibition of HIF1α and LDHA
نویسندگان
چکیده
منابع مشابه
Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes
In the past 6 years, multiple laboratories have reported the successful directed cardiomyocyte differentiation from human pluripotent stem cells (hPSCs; reviewed in Burridge et al). These stem cell–derived cardiomyocytes beat spontaneously, express the expected sarcomeric proteins and ion channels, and exhibit cardiac-type action potentials and calcium transients. They show similar functional p...
متن کاملMitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes
Human pluripotent stem cells derived cardiomyocytes (PSC-CMs) have been widely used for disease modeling, drug safety screening, and preclinical cell therapy to regenerate myocardium. Most studies have utilized PSC-CM grown in vitro for a relatively short period after differentiation. These PSC-CMs demonstrated structural, electrophysiological, and mechanical features of primitive cardiomyocyte...
متن کاملMetabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes
Cardiovascular disease is a leading cause of death worldwide. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold immense clinical potential and recent studies have enabled generation of virtually pure hPSC-CMs with high efficiency in chemically defined and xeno-free conditions. Despite these advances, hPSC-CMs exhibit an immature phenotype and are arrhythmogenic in vivo, necessi...
متن کاملMaturation of pluripotent stem cell derived cardiomyocytes: The new challenge
Stem cell therapy appears to be a promising area of research for cardiac regeneration following ischemic heart failure. However, in vitro differentiation of cardiomyocytes from pluripotent stem cells, or directly from somatic cells, leads to generation of "immature" cardiomyocytes that differ from their adult counterparts in various ways. This immaturity triggers some challenges for their poten...
متن کاملMechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes.
BACKGROUND Human embryonic stem cells (hESCs) can be efficiently and reproducibly directed into cardiomyocytes (CMs) using stage-specific induction protocols. However, their functional properties and suitability for clinical and other applications have not been evaluated. METHODS AND RESULTS Here we showed that CMs derived from multiple pluripotent human stem cell lines (hESC: H1, HES2) and t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Circulation Research
سال: 2018
ISSN: 0009-7330,1524-4571
DOI: 10.1161/circresaha.118.313249